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MB Sample ID: SA016017
| Local Sample ID: | A_0276 |
| Subject ID: | SU000382 |
| Subject Type: | human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Age Or Age Range: | 27-61 years |
| Gender: | male/female |
| Human Race: | caucasian, hispanic, african american |
| Species Group: | Human |
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Collection:
| Collection ID: | CO000376 |
| Collection Summary: | For extraction, 500 µL of an ice-cold solution of 0.005 mg/mL Tryptohan-d5 in 90:10 Methanol:Chloroform (v/v) was added to the tubes containing the thawed human dermal fibroblasts cell pellets. MagNA Lyser ceramic beads were added to the tubes, and a MagNA Lyser was used to beat the samples for two thirty seconds pulses at 2,000 rpm, placing the samples on cold block for 5 minutes in between pulses. Sonication for five minutes was performed to the beaten cell samples, followed by centrifugation at room temperature and at 16,000 rcf for 4 minutes. A 300 µL aliquot of each experimental cell sample homogenized supernatant was transferred to new pre-labeled 2.0 mL LoBind Eppendorf tubes and stored at -80 °C for one hour. |
| Sample Type: | fibroblasts |
