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MB Sample ID: SA028904

Local Sample ID:HC2_3
Subject ID:SU000582
Subject Type:Cells
Subject Species:Homo sapiens
Taxonomy ID:9606
Cell Strain Details:Suzuki, H., Moldoveanu, Z., Hall, S., et al. IgA1-secreting cell lines from patients with IgA nephropathy produce aberrantly glycosylated IgA1. J Clin Inv. 2008, 118, 629-639
Cell Primary Immortalized:immortalized
Cell Counts:1x10^7 cells / pellet
Species Group:Human

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Collection:

Collection ID:CO000576
Collection Summary:An aliquot of cells (1 x 10^7) grown in suspension culture was transferred into a 15 mL conical tube containing 4x the culture volume of ice-cold 0.9% (w/v) NaCl. Cells were pelleted for 5 minutes at 1,500 rpm in a 4oC refrigerated centrifuge. Supernatant was removed, and the pellet was gently resuspended in 1 mL of ice-cold 0.9% (w/v) NaCl to wash and transfer into a 2 mL Lo-Bind microcentrifuge tube (Eppendorf, #022431102). Cells were pelleted for 3 minutes at 1,300 rcf in a 4oC refrigerated centrifuge. The entire supernatant was removed and pellets were snap-frozen in liquid nitrogen and immediately stored at -70°C.
Sample Type:Cell pellets
Storage Conditions:-80 C
Tissue Cell Quantity Taken:1 x 10^7
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