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MB Sample ID: SA032260
| Local Sample ID: | AMD04_006 |
| Subject ID: | SU000613 |
| Subject Type: | HUman |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Age Or Age Range: | 51-95 |
| Gender: | 253 female; 143 male |
| Human Smoking Status: | 5 smokers; 113 ex-smokers; 275 non smokers |
| Human Inclusion Criteria: | For AMD group: clinical diagnosis of AMD, confirmed on color fundus photographs; For controls: age equal or superior than 50 years old, with normal macular health |
| Human Exclusion Criteria: | Diagnosis of any other vitreoretinal disease, active uveitis or ocular infection, significant media opacities that precluded the observation of the ocular fundus, refractive error equal or greater than 6 diopters of spherical equivalent, past history of retinal surgery, history of any ocular surgery or intra-ocular procedure (such as laser and intra-ocular injections) within the 90 days prior to enrolment, and diagnosis of diabetes mellitus, with or without concomitant diabetic retinopathy. |
| Species Group: | Human |
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Collection:
| Collection ID: | CO000607 |
| Collection Summary: | For all participants, fasting blood samples were collected into sodium-heparin tubes, and centrifuged within 30 min (1500 rpm, 10 min, 20ºC). Plasma aliquots of 1.5 mL (MEE) and 5 mL (FMUC/AIBILI) were transferred into sterile cryovials and stored at -80ºC. Plasma samples from MEE were shipped to Portugal for metabolomic profiling in dry ice (through TNT Express, USA, INC). Samples arrived frozen in less than 48 hours and were immediately stored at -80ºC until NMR analysis. |
| Sample Type: | Blood plasma |
| Collection Method: | For all participants, fasting blood samples were collected into sodium-heparin tubes, and centrifuged within 30 min (1500 rpm, 10 min, 20ºC). Plasma aliquots of 1.5 mL (MEE) and 5 mL (FMUC/AIBILI) were transferred into sterile cryovials and stored at -80ºC. Plasma samples from MEE were shipped to Portugal for metabolomic profiling in dry ice (through TNT Express, USA, INC). Samples arrived frozen in less than 48 hours and were immediately stored at -80ºC until NMR analysis. |
| Collection Location: | Retina Service, Massachusetts Eye and Ear, Harvard Medical School, Boston, US; Faculty of Medicine, University of Coimbra, Coimbra, Portugal; Association for Innovation and Biomedical Research on Light and Image (AIBILI), Coimbra, Portugal. |
| Collection Frequency: | one collection |
| Volumeoramount Collected: | 9 tp 10 mL |
| Storage Conditions: | Plasma aliquots were stored at -80ºC. Plasma samples from MEE were shipped to Portugal for metabolomic profiling in dry ice (through TNT Express, USA, INC). Samples arrived frozen in less than 48 hours and were immediately stored at -80ºC until NMR analysis. |
| Collection Vials: | sodium-heparin tubes |
| Storage Vials: | sterile cryovials |
| Collection Tube Temp: | room temperature |
| Additives: | No |
| Blood Serum Or Plasma: | Yes, blood samples were collected, which were then centrifuged to obtain plasma |
