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MB Sample ID: SA045313
| Local Sample ID: | Tgon_HKKO_13Cglutamine_t005_B |
| Subject ID: | SU000843 |
| Subject Type: | Cells (protozoan parasite) |
| Subject Species: | Toxoplasma gondii |
| Taxonomy ID: | 5811 |
| Genotype Strain: | RH (Type I strain) |
| Cell Biosource Or Supplier: | ATCC |
| Subject Comments: | Asexual stage (Tachyzoite) |
| Cell Passage Number: | In continuous culture |
| Cell Counts: | 10^8 cells per sample |
| Species Group: | Microorganism |
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Collection:
| Collection ID: | CO000837 |
| Collection Summary: | Extracellular tachyzoite stage Toxoplasma gondii parasites incubated in either +13C6-glucose or +13C5-15N2-glutamine containing DMEM medium for 0, 5, 10, 30, 60 & 120 minutes were collected by centrifugation and immediately extracted with chilled acetonitrile:water (80:20) mixture and further process for LC-MS analysis. |
| Sample Type: | Cells |
| Collection Method: | Centrifuging cell suspension at 5000 rpm for 5 min at 4 deg. Cel. |
| Collection Time: | At 0, 5, 10, 30, 60 & 120 minutes post labeling with either +13C6-glucose or +13C5-15N2-glutamine. |
| Volumeoramount Collected: | 10^8 cells per sample |
| Storage Conditions: | -80 deg. Cel. Freezer post extraction until LC-MS analysis can be carried out. |
| Collection Vials: | 1.5 ml capped tubes |
| Storage Vials: | 1.5 ml capped tubes |
| Collection Tube Temp: | 4 deg. Cel. |
