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MB Sample ID: SA053797
| Local Sample ID: | 2N |
| Subject ID: | SU000952 |
| Subject Type: | NMR based metabolomics of microbes |
| Subject Species: | Microbacterium sediminis |
| Taxonomy ID: | 904291 |
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Collection:
| Collection ID: | CO000946 |
| Collection Summary: | A psychrotolerant strain of Microbacterium sediminis numbered as YLB-01 isolated from deep sea sediment of the Indian Ocean was used in this study. The strain can grow at temperatures ranging from 4 °C to 50 °C with an optimal growth temperature of 28 °C (Yu et al. 2013). Tryptone soy broth (TSB) medium, which contains 15 g/L tryptone, 5 g/L soy peptone, and 5 g/L NaCl, was used to cultivate the strain. The strain was maintained in glycerol tubes under -80 °C in a refrigerator and was activated using streaking inoculation on agar plates before use. For metabolomics research, a single colony was first inoculated from an agar plate to 32 test tubes containing 5 mL of TSB medium each and cultivated using a shaker under 28 °C for 12 hours. These seed cultures were then transferred to 150 mL Erlenmeyer flasks containing 100 mL of TSB medium each. In order to obtain a sufficient amount of cells, these cells were cultivated under normal temperature (28 °C) and atmospheric pressure (0.1 MPa) until logarithmic or stationary phase before transferred to lower temperature. Specifically, half of these samples were cultivated using a shaker under 28 °C for 18 hours to reach mid logarithmic phase (assigned as scenario L) and the other half were cultivated for 24 hours to reach stationary phase (assigned as scenario S) under the same condition. All samples were then transferred into 100 mL normal saline bags and put into a water-filled high-pressure chamber with the pressure set at 30 MPa. For half of the samples from a certain scenario (L or S) the temperature of the chamber was set at 28 °C. For the other half of the samples the temperature of the chamber was set at 4 °C. All samples were harvested after 7 days. The samples were grouped as NT-L, LT-L, NT-S, and LT-S based on their different cultivation conditions and sampling time |
| Sample Type: | cells |
