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MB Sample ID: SA069526
Local Sample ID: | 166_FP_24 |
Subject ID: | SU001077 |
Subject Type: | Human |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Human Inclusion Criteria: | Caesarean delivery, Non-labored, Full term |
Human Exclusion Criteria: | Twins |
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Collection:
Collection ID: | CO001071 |
Collection Summary: | The placentas were collected immediately following cesarean delivery, and the tissue specimens were collected from the whole placenta at five time points postdelivery: (1) < 5 min (n = 6); (2) 15 min (n = 13); (3) 30 min (n = 13); (4) 1 h (n = 13); (5) 24 h (n = 13). The tissue specimens were immediately frozen in liquid nitrogen following collection. The whole placentas were kept at 4 °C between sampling time points. Tissue specimens were collected from two areas of the maternal (approximately 1 × 1 cm area; depth of 1 cm) and fetal surfaces (approximately 1 × 1 cm area; depth of 0.3 cm) of each placenta at each time point, resulting in 24 total specimens for the < 5 min time point and 52 total specimens for all other time points. Tissue specimens were immediately frozen in liquid nitrogen following collection and stored at −80 °C until metabolomic analysis |
Sample Type: | Placenta |
Collection Method: | Tissue specimens were collected from two areas of the maternal (approximately 1 × 1 cm area; depth of 1 cm) and fetal surfaces (approximately 1 × 1 cm area; depth of 0.3 cm) of each placenta at each time point by cutting with sterile scalpel and forceps on a sterile surface. The tissue specimens were immediately frozen in liquid nitrogen following collection. |
Collection Frequency: | Tissue specimens were collected from the whole placenta at five time points postdelivery: (1) < 5 min (n = 6); (2) 15 min (n = 13); (3) 30 min (n = 13); (4) 1 h (n = 13); (5) 24 h (n = 13) |
Storage Conditions: | -80℃ |
Collection Vials: | Nunc 1.8mL Cryogenic tubes (ThermoFisher) |
Storage Vials: | Nunc 1.8mL Cryogenic tubes (ThermoFisher) |