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MB Sample ID: SA074557
| Local Sample ID: | 12C-AC-Leaf |
| Subject ID: | SU001139 |
| Subject Type: | Plant |
| Subject Species: | Allium cepa;Arabidopsis thaliana;Glycine max;Glycyrrhiza glabra |
| Subject Comments: | Allium cepa/Arabidopsis thaliana/Glycine max/Glycyrrhiza glabra/Glycyrrhiza urahensis/Medicago truncatula/Nicotiana tabacum/Ophiorrhiza pumila/Oryza sativa/Solanum lycopersicum/Solanum tuberosum/Zea mays |
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Collection:
| Collection ID: | CO001133 |
| Collection Summary: | Leaf/stem and root tissues of Arabidopsis thaliana, Nicotiana tabacum, Glycine max, Glycyrrhiza glabra, Glycyrrhiza urahensis, and Oryza sativa, bulb, leaf, and root tissues of Allium cepa, flower, leaf/stem, seed pod, seed, and root tissues of Medicago truncatula, green and ripe fruit-, and leaf/stem tissues of Solanum lycopersicum, leaf/stem, root, and tuber tissues of Solanum tuberosum, and leaf, stem, seed, and root tissues of Zea mays were purchased from IsoLife Co. (Wageningen UR, Netherlands). The hairy root of Ophiorrhiza pumila was grown as follows. O. pumila hairy roots were derived and maintained, except for the carbon source. We supplied 1.5% D-glucose instead of 1.5% sucrose. We obtained 4-week-old O. pumila hairy roots and sub-cultured them in 1.5% D-glucose (U-13C6, 99%) (Cambridge Isotope Laboratories, Inc.) for two generations for complete isotope labeling of the metabolome. Hairy roots from the same batch used for isotope-labeling were sub-cultured for two subcultures in 1.5% D-glucose (Nacalai Tesque, Inc); they served as the control. Hairy roots from the second sub-culture of sub-cultured tissues growing in 1.5% isotope-labelled or un-labelled 1.5% D-glucose were harvested, wiped with paper towels and snap-frozen in liquid-N2. |
| Sample Type: | Plant |
