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MB Sample ID: SA076769
| Local Sample ID: | 12-Late Exponential Phase |
| Subject ID: | SU001169 |
| Subject Type: | Bacteria |
| Subject Species: | Acidithiobacillus ferrooxidans ATCC 23270 |
| Taxonomy ID: | 243159 |
| Genotype Strain: | ATCC 23270 |
| Cell Biosource Or Supplier: | American Type Culture Collection |
| Cell Strain Details: | ATCC 23270 |
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Collection:
| Collection ID: | CO001163 |
| Collection Summary: | Replicate cultures of Acidithiobacillus ferrooxidans (American Type Culture Collection strain 23270) were grown in 1 L of Tuovinen and Kelly (1973) liquid medium ([FeSO4·7H2O] = 33.4 g/L; 0.3 % (w/v) (NH4)2SO4; MgSO4·7H2O; K2HPO4, pH 2.5). Cells were incubated on a gyratory shaker at 180 rpm and 30 °C. Cells harvested for the metabolomic comparison of two points in the growth curve were in late exponential growth phase (OD425 = 0.85) or in early exponential growth phase (OD425 = 0.65). The cells were harvested via centrifugation at 14,000 x g for 5 min at 4 °C then transferred into a 1.5 mL centrifuge tube. |
| Sample Type: | Bacterial cells |
