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MB Sample ID: SA079582
| Local Sample ID: | 13 |
| Subject ID: | SU001210 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Genotype Strain: | C57BL6 |
| Age Or Age Range: | 2-3 months |
| Weight Or Weight Range: | 28-46 |
| Gender: | Female |
| Animal Animal Supplier: | Jackson Laboratory |
| Animal Housing: | Single House |
| Animal Light Cycle: | 12:12 |
| Animal Feed: | CHOW diet (Envigo Teklad 6% fat 7002, Madison, WI) and Western (HFD; high-fat, high-sugar; 21% milk fat and 34% sucrose (Envigo TD.08811) |
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Collection:
| Collection ID: | CO001204 |
| Collection Summary: | Approximately 20 mg of muscle tissue was homogenized in a glass homogenizer with 1.5 ml of 2:1 chloroform:methanol and then brought to 4 ml using the same ratio. The mixture was poured through a 2V grade qualitative 12.5 cm Whatman filter into a clean 10 ml glass tube. The volume in the tube was again brought up to 4 ml with the same 2:1 solution as above. One ml of water was added to the tube, vortexed for 20 seconds, and then centrifuged for 10 minutes at 2500 rpm. The top non-lipid portion was removed and the lower lipid-containing layer was dried under nitrogen. |
| Sample Type: | Muscle |
| Collection Method: | excision |
| Collection Location: | femoral muscle |
| Storage Conditions: | -80℃ |
