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MB Sample ID: SA094107
Local Sample ID: | 233_15 |
Subject ID: | SU001368 |
Subject Type: | Human |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Age Or Age Range: | 32-67 |
Gender: | Male and female |
Human Race: | Caucasian (C) and African American (AA) |
Human Ethnicity: | non-hispanic |
Human Inclusion Criteria: | Sepsis: 1) Suspected or confirmed infection; 2) Any two of four criteria of systemic inflammatory response in ED(18); 3) Age ≥ 18; 4) Lactate ≥ 2.0 mmol/L; 5) Enrollment within 2 hours of initiation of quantitative resuscitation protocol. Controls: admitted to the emergency department and had no medical conditions that required chronic administration of medication expected to affect platelet function (aspirin, PGY12 inhibitors, etc) |
Human Exclusion Criteria: | Exclusion criteria were:1) any primary diagnosis other than sepsis; 2) established Do Not Resuscitate status; 3) transferred from another hospital with sepsis therapy already initiated; 4) cardiopulmonary resuscitation (chest compression or defibrillation) prior to enrollment; 5) patient or legal representative unable to understand and sign informed consent. |
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Collection:
Collection ID: | CO001363 |
Collection Summary: | WB samples (12 mL each) were collected by direct venipuncture or from an indwelling line into K2 EDTA-containing tubes. Platelets were then isolated. centrifuged (200x gfor 6 min at room temperature).The platelet-rich plasma layer was transferred to another tube and centrifuged (4500x gfor 5 minat 4C) to pellet the platelets.The nearly cell-free plasma layer was transferred to a new tube, leaving approximately 0.25 mL of plasma in the original tube. Remaining plasma was used to resuspend the platelet pellet to produce ultra-rich plasma. |
Sample Type: | Platelets |
Collection Method: | direct venipuncture or indwelling line |
Collection Frequency: | once |
Volumeoramount Collected: | 12mL |
Storage Conditions: | Described in summary |
Collection Vials: | K2 EDTA-containing Vacutainer tubes (BD 134367863; Becton-Dickinson, Franklin Lakes, NJ USA) |
Additives: | K2 EDTA |