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MB Sample ID: SA116247
| Local Sample ID: | F_20m_4b |
| Subject ID: | SU001489 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Genotype Strain: | C57BL/6J |
| Age Or Age Range: | 4 months & 20 months |
| Gender: | Male and female |
| Animal Animal Supplier: | Jackson Laboratories |
| Animal Housing: | SPF animal facility at USC |
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Collection:
| Collection ID: | CO001484 |
| Collection Summary: | The hindlimb bones of each mouse were harvested and kept on ice in D-PBS (Corning) supplemented with 1% Penicillin/Streptomycin (Corning) until further processing. Muscle tissue was removed from the bones, and the bone marrow from cleaned bones was collected into clean tubes (Amend et al., 2016). Red blood cells from the marrow were removed using Red Blood Cell Lysis (Miltenyi Biotech #130-094-183), according to the manufacturer’s instructions, albeit with no vortexing step to avoid unscheduled neutrophil activation. Neutrophils were isolated from other bone marrow cells using magnetic-assisted cell sorting (Miltenyi Biotech kit #130-097-658). Viability and yield were assessed using trypan blue exclusion and an automated COUNTESS cell counter (Thermo-Fisher Scientific). Purified cells were pelleted at 300g and snap-frozen in liquid nitrogen until processing for RNA, lipid or metabolite isolation. |
| Sample Type: | Bone marrow |
