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MB Sample ID: SA120127
Local Sample ID: | Staphep_TSB_3 |
Subject ID: | SU001500 |
Subject Type: | Bacteria |
Subject Species: | Staphylococcus epidermidis |
Taxonomy ID: | 1282 |
Genotype Strain: | ATCC 12600; ATCC 12228 |
Cell Biosource Or Supplier: | ATCC |
Cell Strain Details: | ATCC 12600; ATCC 12228 |
Select appropriate tab below to view additional metadata details:
Collection:
Collection ID: | CO001495 |
Collection Summary: | Staphylococcus aureus (ATCC 12600) and Staphylococcus epidermidis (ATCC 12228) were cultured in four filter-sterilized complex media for metabolomics analysis: Brain Heart Infusion broth (BHI; Bacto); lysogeny broth Lennox (LB; Fisher Scientific); Mueller Hinton broth (MHB; Difco); and Tryptic Soy broth (TSB; Bacto). Each species was prepared in biological triplicate. Uninoculated media control blanks were prepared in six replicates for BHI, LB, and MHB, and in triplicate for TSB, following the same procedures as the bacterial samples and processed in parallel. Samples were transferred to capped GC vials and stored at -20 °C for approximately two weeks prior to GC×GC-TOFMS analysis. |
Collection Protocol Filename: | CLJenkins_Collection_Protocol_Metadata.txt |
Sample Type: | Bacterial cells |
Collection Frequency: | Once, at the completion of 24 hour aerobic incubation at 37 C with orbital shaking |