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MB Sample ID: SA128430
| Local Sample ID: | Cell_9312_A |
| Subject ID: | SU001598 |
| Subject Type: | Other |
| Subject Species: | Prochlorococcus marinus str. MIT 9312;Prochlorococcus marinus MIT9313 |
| Taxonomy ID: | 74546;74547 |
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Collection:
| Collection ID: | CO001593 |
| Collection Summary: | Axenic cultures of Prochlorococcus strain MIT9312 and MIT9313 were grown in defined artificial AMP1 media supplemented with 10 mM (final concentration) filter-sterilized sodium bicarbonate. Seven 20 L cultures were grown for each of the two Prochlorococcus strains, providing three replicates for the lipid and small metabolite analysis and an additional sample for proteomics analysis. Strains MIT9312 and MIT9313 are available from the National Center for Marine Algae and Microbiota. 20 L cultures were grown in polycarbonate carboys (ThermoFisher Nalgene, Waltham, MA, USA) with gentle stirring (60 rpm), under constant light flux (10 – 20 µmol Q m -2 s -1 for MIT9313; 30 – 40 µmol Q m -2 s -1 for MIT9312) at 24°C. Vesicles were collected from 20 L cultures of Prochlorococcus during mid-to-late exponential growth phase and isolated as described previously (Biller et al., 2014, Science). Briefly, cultures were first gravity filtered through a 0.2 µm capsule filter (Polycap 150TC; GE Life Sciences/Whatman, Maidstone, UK). The filtrate was then concentrated using a 100 kDa tangential flow filter (Ultrasette with Omega membrane; Pall, Port Washington, NY, USA) and re-filtered through a 0.2 µm syringe filter. Vesicles were pelleted from the sample by ultracentrifugation at ~100,000 x g (Beckman-coulter SW32Ti rotor; 32,000 rpm, 1.5 hrs, 4°C), purified on an OptiPrep gradient (Biller et al., 2014, Science), then washed and resuspended in 0.2 µm filtered 1x PBS. |
| Sample Type: | Cultured Prochlorococcus cells and vesicles |
| Storage Conditions: | -80℃ |
