Return to study ST001657 main page
MB Sample ID: SA151932
| Local Sample ID: | 15s_1 |
| Subject ID: | SU001734 |
| Subject Type: | Bacteria |
| Subject Species: | Escherichia coli |
| Taxonomy ID: | 562 |
| Genotype Strain: | K-12 W3110 |
Select appropriate tab below to view additional metadata details:
Collection:
| Collection ID: | CO001727 |
| Collection Summary: | E. coli strain K-12 W3110 (ATCC 27325) was cultured on TSA agar medium. A single bacterial colony was picked from TSA agar medium, and then used to inoculate Luria-Bertani broth (EMD Millipore, Billerica, MA). After being incubated at 37 °C for 12 h on a rotary shaker set to 200 rpm, E. coli cells were harvested at an optical density (OD600) of 1, and then centrifuged at 7,500 × g for 10 min in 50 mL centrifuge tubes. After decanting the supernatant, the pellet was washed with phosphate buffered saline (PBS), and then re-suspended to the volume of bacterial culture for further treatment and analysis. |
| Collection Protocol Filename: | Cell_culture_IPL_treatment_Sample_preparation_method.docx |
| Sample Type: | Bacterial cells |
