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MB Sample ID: SA164943
| Local Sample ID: | S5R1 |
| Subject ID: | SU001853 |
| Subject Type: | Cultured cells |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Gender: | Female |
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Collection:
| Collection ID: | CO001846 |
| Collection Summary: | At the endpoint of the experiment, cells were washed gently with 150 mM ammonium acetate buffer and scraped quickly with a rubber-tipped cell scraper. Subsequently, 500 uL chilled 80% methanol was added and the scraped cells were sonicated using a bioruptor (Diagenode, Philadelphia, PA, USA) in 4 °C water bath during 10 cycles (30s on and 30s off). After that, the sample was placed at -20 °C for 3 h, followed by 10 min of centrifugation at 14000 g at 4 °C. The supernatant containing the metabolite was collected, dried, and stored at -80 °C until LC-HRMS analysis. |
| Sample Type: | HEK cells |
