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MB Sample ID: SA169028
Local Sample ID: | LPIAT1 KO #2-1 |
Subject ID: | SU001894 |
Subject Type: | Cultured cells |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Age Or Age Range: | 8 weeks old |
Gender: | Male |
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Collection:
Collection ID: | CO001887 |
Collection Summary: | MEFs were maintained in Dulbecco’s modified Eagle’s medium (DMEM; Sigma-Aldrich) supplemented with 10% fetal calf serum, 100 units/ml penicillin, 100 µg/ml streptomycin, and 2 mM L-glutamine. 1 × 106 cells were seeded on cell culture dishes (6.0 cm diameter) and the next day, cells were washed twice with PBS and collected to a safe-lock poly-propylene tube (2 ml) with 1 M HCl (500 μl), followed by centrifugation (15,000 g, 5 min at 4 ºC). Supernatants were removed rapidly and resuspended with 750 μl of quench mix, 170 μl of H2O. |
Sample Type: | Fibroblasts |