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MB Sample ID: SA169056
| Local Sample ID: | WT_EBSS_Baf2 |
| Subject ID: | SU001895 |
| Subject Type: | Cultured cells |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Gender: | Not applicable |
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Collection:
| Collection ID: | CO001888 |
| Collection Summary: | MEFs were maintained in Dulbecco’s modified Eagle’s medium (DMEM; Sigma-Aldrich) supplemented with 10% fetal calf serum, 100 units/ml penicillin, 100 µg/ml streptomycin, and 2 mM L-glutamine. For autophagy induction, 2 × 105 cells were seeded on 6-well plates and the next day, cells were washed twice with PBS and incubated in amino acid-free EBSS (starvation medium; Thermo Fisher Scientific 24010-043) in the presence or absence of bafilomycin A1 (Selleck Chemicals) for 30 min at 37 ºC. After the incubation, cells were washed twice with PBS and collected to a safe-lock poly-propylene tube (2 ml) with 1 M HCl (500 μl), followed by centrifugation (15,000 g, 5 min at 4 ºC). Supernatants were removed rapidly and resuspended with 750 μl of quench mix, 170 μl of H2O. |
| Sample Type: | Fibroblasts |
