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MB Sample ID: SA194774
Local Sample ID: | 0412_CD27+_Labeled_2 |
Subject ID: | SU002153 |
Subject Type: | Cultured cells |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Age Or Age Range: | 6-8 weeks old |
Weight Or Weight Range: | 15-20 grams |
Gender: | Male and female |
Cell Strain Details: | C57Bl/6 |
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Collection:
Collection ID: | CO002146 |
Collection Summary: | polarized CD27+ and CD27- γδ T cells were cultured in glucose-free DMEM supplemented with 10 mM [U-13C]-glucose and 10% dialyzed FBS for 24 h. The cells were rinsed in cold PBS and quenched using a mixture containing 2 mL acetonitrile and 1.5 mL H2O. After adding 1 mL chloroform, the sample was homogenized and centrifuged at 3,000 rpm, 4 oC for 20 min. The top layer was transferred to a new tube and lyophilized. The dried sample was then dissolved in 100 µL 20% acetonitrile and vigorously vortex-mixed for 3 min. After centrifugation at 14,000 rpm, 4oC for 20 min, the supernatant was collected for two-dimensional liquid chromatography-mass spectrometry (2DLC-MS) analysis. |
Sample Type: | T-cells |