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MB Sample ID: SA205781
| Local Sample ID: | shchrebp HG-3 |
| Subject ID: | SU002230 |
| Subject Type: | Cultured cells |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
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Collection:
| Collection ID: | CO002223 |
| Collection Summary: | Conditionally immortalized mouse podocytes were cultured as previously reported.25, 35 Podocytes were treated with 20μM of DL-α-palmitin (Sigma-Aldrich), 1-O-Hexadecyl-rac-glycerol (16:0-AG, Santa Cruz) ,1-O-Octadecyl-rac-glycerol (18:0-AG, Sigma-Aldrich) or 0.05% ethanol (vehicle) for 48 hours. An engineered miR-30 based ChREBP knockdown (KD) construct was assembled by PCR using previously validated ChREBP shRNA clone,34 followed by an optimized miR-E backbone. Stable podocyte cell line with ChREBP KD were generated by transfection into constructs together with PiggyBac transposase. After selection with 0.5 μg/ml of blasticidin alone or in combination with 1μg/ml puromycin, GFP-positive cells were sorted by FACS, and the top 30% population were collected. |
| Sample Type: | Epithelial cells |
