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MB Sample ID: SA205979
| Local Sample ID: | QC_2_B |
| Subject ID: | SU002236 |
| Subject Type: | Cultured cells |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
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Collection:
| Collection ID: | CO002229 |
| Collection Summary: | HEK-293T cells were obtained from the ATCC (CRL-11268) and human Jurkat leukemia CD4+ cells were kindly donated by Dr. J. Alcamí (Centro Nacional de Microbiología, Instituto de Salud Carlos III, Madrid, Spain). When needed, Jurkat cells lacking endogenous CXCR4 expression (Jurkat-/-) were transiently transfected with CXCR4-AcGFP (20 µg; JK-/-X4) using a BioRad electroporator (20 × 106 cells/400 µL RPMI 1640 with 10% fetal calf serum) and analyzed 24 hours later. Human peripheral blood mononuclear cells were isolated from buffy coats by centrifugation through FicollPaque PLUS density gradients (GE Healthcare, Wakuesha, WI) at 760 × g for 30 minutes at room temperature (RT). They were then in vitro activated with 20 U/mL of IL-2 (Teceleukin; Roche, Nutley, NJ) and 5 µg/mL phytohemagglutinin PHA (Roche) to generate T cell blasts. |
| Sample Type: | HEK cells |
