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MB Sample ID: SA210642
| Local Sample ID: | T3.30.LWS2_pos |
| Subject ID: | SU002284 |
| Subject Type: | Plant |
| Subject Species: | Pinus pinaster |
| Taxonomy ID: | 71647 |
| Age Or Age Range: | one-two years |
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Collection:
| Collection ID: | CO002277 |
| Collection Summary: | Plantlets were sampled on the first-day assay (T0) under well-watered conditions before the temperature change in both chambers. The following day to T0, the exposure to combined stress began. HWS plantlets were watering with 25 % of the weight loss each day, while LWS plantlets with 50 %. Afterward, heat-stressed and water-stressed plantlets were sampled at the end of the 6-h heat exposure on day 1 (T1), day 3 (T3), day 5 (T5), and day 7 (T7). The water deficit more or less severe was imposed for seven days by progressively depleting soil water content. Immediately after sampling, cell membrane damage and leaf water status were measured in fresh needles by quantifying relative EL and RWC (see below). Other needles were frozen in liquid nitrogen, lyophilized, and stored in the dark and cold (-20 ºC) until use. |
| Sample Type: | Plant |
| Storage Conditions: | -20℃ |
