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MB Sample ID: SA211950
| Local Sample ID: | fed_fasted |
| Subject ID: | SU002305 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
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Collection:
| Collection ID: | CO002298 |
| Collection Summary: | C57BL/6J (000664) and BALB/cJ (000651) mice were obtained from The Jackson Laboratory. Mice were housed at 20-22°C on a 12 h light/dark cycle with ad libitum access to food (PicoLab Rodent Diet 5053) and water. All animal studies were performed in accordance with Haigis lab protocols approved by the Standing Committee on Animals, the Institutional Animal Care and Use Committee at Harvard Medical School. For heat inactivation studies, 3 mice were used (C57BL/6J, female, 7 weeks old) and kidneys, brain halves, and liver lobes from the same individual animal were subjected to the different heat inactivation treatments (overview in Supplementary Fig. 1A, E). For desiccation experiments, 2 mice were used (C57BL/6J, male, 7 weeks old). For fasting experiments, two independent cohorts of 5 mice were used per treatment group (BALB/cJ, female, 10-11 weeks old) and mice were subjected to a 16 hour overnight fast. For HFD experiments, two independent cohorts of 4 mice were used per treatment group (C57BL/6J, female). Mice were assigned at 5 weeks old to the control diet (PicoLab Rodent Diet 5053) or HFD (Research Diets, Inc. #12492) and maintained on this diet for 4.5 months. The control diet is 4.07 Gross Energy Kcal/g. The HFD is 5.21 Kcal/g. for 8-10 weeks. Comparative MALDI MSI and LC-MS analyses of tissues were always performed on the same tissue specimens. |
| Sample Type: | Liver_Brain_Kidney |
