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MB Sample ID: SA217821
| Local Sample ID: | B01-02_60_1_1704 |
| Subject ID: | SU002359 |
| Subject Type: | Cultured cells |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Gender: | Not applicable |
| Cell Biosource Or Supplier: | Sigma-Aldrich (St. Louis, MO) |
| Cell Strain Details: | Hep3B |
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Collection:
| Collection ID: | CO002352 |
| Collection Summary: | Cell lines and culture conditions: The Hep3B cell line used in this study was purchased from Sigma-Aldrich (St. Louis, MO). Sorafenib-resistant Hep3B cells were developed from Hep3B cell lines One million Hep3B cells were seeded in T75 flasks and cultured in Dulbecco's Modified Eagle Medium (DMEM) as monolayers. The medium was sup-plemented with 10% fetal bovine serum (FBS) (Sigma Aldrich, St. Louis, #F2442-500ML) and 1% penicillin/streptomycin (Sigma Aldrich, St. Louis, #P4333). Cultures were then incubated in 5% CO2 humidified atmosphere at 37°C for 3-4 days until 70-80% confluency was reached. Aseptic techniques were applied to avoid poten-tial contamination, and the confluency and contamination were checked routinely. |
| Sample Type: | Cultured cells |
