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MB Sample ID: SA235461
Local Sample ID: | MDRP1-2-PE |
Subject ID: | SU002435 |
Subject Type: | Cultured cells |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606/10090 |
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Collection:
Collection ID: | CO002428 |
Collection Summary: | Fibroblasts and MEFs were cultured under 5% CO2 at 37°C in Dulbecco's modified medium (DMEM; Invitrogen, Carlsbad, CA) supplemented with 10% fetal bovine serum. For the extraction of phospholipids, fibroblasts were seeded in 6-well plate and cultured overnight, followed by replacement of fresh culture medium. Cells were harvested at 48 hours after the medium change as described below. |
Sample Type: | Cultured fibroblasts |