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MB Sample ID: SA235724
| Local Sample ID: | RS_M6-1_1 |
| Subject ID: | SU002439 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Genotype Strain: | C57BL/6J |
| Age Or Age Range: | 6-8 weeks |
| Gender: | Female |
| Animal Animal Supplier: | The Jackson Laboratory, Bar Harbor, ME |
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Collection:
| Collection ID: | CO002432 |
| Collection Summary: | Female C57BL/6J mice (The Jackson Laboratory, Bar Harbor, ME) 6-8 weeks of age were used for these studies. Mice were housed according to NIH guidelines for housing and care in a biosafety level 3 animal laboratory. All procedures were approved by the Institutional Animal Care and Use Committee (protocol number 16-011) of Northern Arizona University. The Coccidioides isolates used in this study were the type strains C. immitis strain RS (ATCC® catalog no. NR-48942; NCBI accession no. AAEC00000000.3) and C. posadasii strain Silveira (ATCC® catalog no. NR-48944; NCBI accession no. ABAI00000000.2). Mice were anesthetized with ketamine/xylene (80/8 mg/kg) and intranasally inoculated with 100 arthroconidia of C. immitis strain RS (n=6) or C. posadasii strain Silveira (n=6) suspended in 30 μL phosphate-buffered saline (PBS), as described previously (22, 58). Control mice were inoculated with PBS alone (n=4). The mice were sacrificed at day 10 post-inoculation. The lungs were rinsed with 2 mL of PBS to collect bronchoalveolar lavage fluid (BALF), which were filtered with 0.22 μm Ultrafree® - MC centrifugal filter devices with Durapore® membrane (MilliporeSigma, Burlington, MA). One milliliter of each BALF sample was stored at –80°C for volatilomics analysis. Halt™ Protease Inhibitor Cocktail (10 μL/mL) was added to the remainder of each BALF sample for cytokine analysis. Spleen and brain were homogenized in 1 ml of sterile PBS followed by culture of 10-fold dilutions of each tissue on 2X GYE agar (2% glucose (VWR™, USA), 1% yeast extract (BD™, Franklin Lakes, New Jersey, USA, and 1.5% bacteriological agar (Difco, USA)) to assess fungal dissemination. |
| Sample Type: | Bronchoalveolar lavage |
