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MB Sample ID: SA240878

Local Sample ID:C18-5-neg
Subject ID:SU002495
Subject Type:Plant
Subject Species:Brassica napus
Taxonomy ID:3708

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Collection:

Collection ID:CO002488
Collection Summary:A total of 27 samples from the three treatments (PC, Si0.5 and Si1.0) were used for metabolomic analyses at 7-, 14- and 21 dpi. Hi-Q seedlings were grown in the soil-mix as previously described with/without Si amendment, inoculated with P. brassicae, and subsequently, washed thoroughly, flash-frozen in liquid nitrogen and stored at -80°C until analysis. Each biological replicate was comprised of 5 individual plants per treatment, and three biological replicates were generated from three independent experiments. A total of 27 samples were generated for transcriptomics and metabolomics analyses [3 treatments (PC, Si0.5 and Si1.0) x 3 time-points (7-, 14- and 21 dpi) x 3 biological replicates].
Collection Protocol Filename:Collection_protocol
Sample Type:Plant
Collection Location:Alberta, Canada
Collection Frequency:7, 14 21 DPI
Volumeoramount Collected:0.07g per sample
Storage Conditions:-80℃
Collection Vials:2 mL round-bottom Eppendorf tubes
Storage Vials:2 mL round-bottom Eppendorf tubes
Collection Tube Temp:-20
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