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MB Sample ID: SA248837
Local Sample ID: | AG-23-TS-32 |
Subject ID: | SU002578 |
Subject Type: | Mammal |
Subject Species: | Mus musculus |
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Collection:
Collection ID: | CO002571 |
Collection Summary: | C57BL/6 mice were orthotopically implanted with PK5L1940 cells. 8 Gy RT was administered 7 days post-implantation. PD1-IL2v and aCD25 dosed once per week beginning day 7 post-implantation. Spleens and lymph nodes were harvested 17 days post implantation and homogenized to a single cell suspension by mashing through a 70 uM cell strainer. For spleen processing, the erythrocytes were lysed with ACK (ammonium-chloride-potassium) lysis buffer for 3 min at RT. CD8 T cells were sorted with a CD8-negative selection Stemcell isolation kit following manufacturer instructions. |
Sample Type: | Cell culture supernatant |