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MB Sample ID: SA252859
| Local Sample ID: | Control day 5.3 |
| Subject ID: | SU002611 |
| Subject Type: | Bacteria |
| Subject Species: | Aspergillus niger/Bacillus subtilis |
| Taxonomy ID: | 5061/1423 |
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Collection:
| Collection ID: | CO002604 |
| Collection Summary: | For analysis and trapping of volatile organic compounds two-compartment glass Petri-dishes (Garbeva et al., 2014) containing 1% LB-A (Carl-Roth LB- Agar) were applied. The fungus Aspergillus niger was inoculated 24 hours before addition of the bacterial strain on the left side of the two compartment glass petri-dish and incubated overnight at 28 °C. The bacterial strain Bacillus subtilis 3610 was incubated overnight at 28 °C in LB- liquid medium (Carl-Roth LB- Medium). After overnight _incubation a volume of 2 µl of the grown Bacillus subtilis 3610 culture was spotted on the opposite side of the Aspergillus niger culture. The glass Petri-dishes were kept open for 25 min. for drying of the droplets. After the drying process plates were closed by a lid with an outlet connected to a steel trap containing 150 mg Tenax TA and 150 mg Carbopack B (Markes International Ltd) and incubated at 28 °C. The Tenax steel traps were collected after three and five days of incubation and stored at 4 °C until GC-Q-TOF analysis. As controls, glass Petri-dishes containing LB agar media without inoculated bacteria or fungi were used. |
| Sample Type: | Fungal/bacterial volatiles |
| Collection Method: | Tenax Trap |
| Collection Location: | Wageningen, The Netherlands |
| Collection Frequency: | 3 days and 5 days |
| Collection Duration: | 5 min. |
| Storage Conditions: | 4℃ |
| Collection Vials: | Tenax |
| Storage Vials: | Metal tubes |
