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MB Sample ID: SA297408
| Local Sample ID: | 2021-09-10 JL74 SOWE4 3x dilution GSH GSSG MRM TR3_1-B |
| Subject ID: | SU002885 |
| Subject Type: | Amphibian |
| Subject Species: | Xenopus laevis |
| Taxonomy ID: | 8355 |
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Collection:
| Collection ID: | CO002878 |
| Collection Summary: | The Spemann-Mangold Organizer (SMO) was lineage-traced under epifluorescence and isolated in a 2% (w/v) agarose-coated Petri dish containing 50% SS. The dissected SMO tissues and the remainder embryo (RE) were collected in LoBind Eppendorf tubes separately, and processed using our established protocols for metabolomic analysis. For MS-based metabolomics of the TCA cycle and glycolysis, 10 dissections of SMO and RE were pooled as 1 BR. For targeted quantification of reduced and oxidized glutathione, 20 tissues of SMO and RE were dissected and pooled as one BR. The dissected tissues were stored in LC-MS-grade methanol at −80 °C until sample processing. |
| Sample Type: | Dissected organizer from Xenopus laevis |
