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MB Sample ID: SA300820
| Local Sample ID: | B54_017 |
| Subject ID: | SU002910 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Genotype Strain: | n/a |
| Age Or Age Range: | 3 weeks |
| Weight Or Weight Range: | not specified |
| Height Or Height Range: | not specified |
| Gender: | Male and female |
| Animal Housing: | regular housing |
| Animal Feed: | regular diet |
| Animal Water: | ad libitum |
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Collection:
| Collection ID: | CO002903 |
| Collection Summary: | Bone marrow cells were isolated as previously reported (35). Briefly, femurs were flushed by 25G5/8 needle with medium containing RPMI1640+2% FBS+10 units/ml Heparin + penicillin and streptomycin. Bits of bones were removed using a sterile 4um nylon cell strainer (Falcon 352340). Cells were dissolved in 50 ml medium and centrifuged at 2000 rpm (900x g), for 10 minutes at 4oC. Cell pellets were then washed twice with 50 ml of serum-free RPMI (RPMI1640+20mM Hepes + penicillin and streptomycin, adjusted to pH 7.4 before filtering), centrifuged at 2000 rpm, at 4oC for 5 minutes, and resuspended in 25 ml of serum-free RPMI. |
| Sample Type: | Bone marrow |
