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MB Sample ID: SA308729
| Local Sample ID: | P_PA-A-5 |
| Subject ID: | SU002966 |
| Subject Type: | Bacteria |
| Subject Species: | Staphylococcus aureus/ Acinetobacter baumannii/ Enterococcus faecium/ Pseudomonas aeruginosa |
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Collection:
| Collection ID: | CO002959 |
| Collection Summary: | All work with microorganisms was performed under Biosafety Level 2 (BSL-2) conditions. Bacteria were streaked onto agar plates from stocks and incubated overnight at 37°C. Single colonies were collected from the agar plates and suspended in sterile deionized (DI) water to a turbidity of 2.0-2.05 McFarlands (equivalent to ca. 6.0 x 108 CFU/mL). Five biological replicates were prepared for each strain. Tryptic Soy Broth was inoculated at a 1:10 dilution (5 mL total volume) and incubated overnight at 37°C with shaking (180 rpm). The cultures were then centrifuged at 2700 rpm for 10 min at 4 °C, after which the broth was discarded. The pelleted bacteria were washed and resuspended in 2 mL of sterile water. |
| Sample Type: | Bacterial cells |
