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MB Sample ID: SA315462
| Local Sample ID: | D1_2 |
| Subject ID: | SU003004 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Age Or Age Range: | 15-20 week old |
| Gender: | Male |
| Species Group: | C57BL/6J |
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Collection:
| Collection ID: | CO002997 |
| Collection Summary: | MI was induced in adult (15-20 week old) male C57BL/6J mice as previously described.2,4,6 Mice were anesthetized (2% isoflurane) and laid supine on a heated stage (37°C), intubated and connected to a mouse ventilator (Harvard Apparatus; 300µL stroke volume, 300 breaths/min). The chest was incised to expose the ribs, and the heart exposed between the 3rd and 4th ribs. The pericardial layer was gently removed, and the left coronary artery was ligated ~1mm below the left atrium using 8-0 suture. Ischemia was confirmed by blanching of the LV. For glutamine administration, mice were injected intraperitoneally with glutamine (0.75-1g/kg) or saline vehicle beginning at either day 1 post-MI (for day 3 time-point) or beginning at day 3 (for day 7 time-point). A separate cohort of mice was injected 2 hr after MI and studied 24 hr later (day 1). To inhibit glutamine metabolism, mice were administered BPTES (12.5mg/kg; 10% in DMSO/corn oil), a glutaminase-1 inhibitor, beginning at either day 1 or day 3. |
| Sample Type: | Macrophages |
