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MB Sample ID: SA318230
Local Sample ID: | bs_r4 |
Subject ID: | SU003045 |
Subject Type: | Mammal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Genotype Strain: | C57BL/6J |
Age Or Age Range: | 20 wks |
Gender: | Male |
Animal Animal Supplier: | Jackson Lab |
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Collection:
Collection ID: | CO003038 |
Collection Summary: | Extraction of fecal metabolites: 50 mg of fresh feces were collected from each mouse and frozen in liquid nitrogen. Samples were spiked with 0.5 mL water, homogenized for 5 min followed by 10 s of sonication, and centrifuged at 14K rpm X 10 min at 4°C. Collected supernatants were evaporated to dryness under a nitrogen gas stream and stored at −80°C. Extraction of brain metabolites: Approximately 20-40 mg of frozen tissues was resuspended in 4 volumes of MPER and homogenized on ice. 130 µL of tissue homogenates were spin filtered, as above, and the flow through was diluted 1:1 with deionized water, followed by sonication for 10 sec using a Sonic Dismembrator on ice, vortexed for 10 s, and centrifuged at 14K rpm X 15 min at 4°C. 100 uL of eluate was evaporated to dryness under a nitrogen gas stream and stored at −80°C. |
Sample Type: | feces, hippocampus, brainstem |
Storage Conditions: | -80℃ |