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MB Sample ID: SA323260
| Local Sample ID: | 4A2-02-10502 |
| Subject ID: | SU003089 |
| Subject Type: | Mammal |
| Subject Species: | BALB/C nude mice |
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Collection:
| Collection ID: | CO003082 |
| Collection Summary: | MDA-MB-231 cell line was cultured in DMEM medium supplemented with 10% fetal bovine serum and 1% penicillin/streptomycin (Sigma Aldrich, St. Louis, MO, USA) at 37 °C in a humidified atmosphere containing 5% CO2. This study was approved by the Institutional Animal Care and Use Committee (IACUC) at the University of Sharjah (Ethical Approval Number: ACUC-06-08-2022). Standard ethical guidelines were followed in all animal procedures conducted. Thirty female BALB/C nude mice (8-10 weeks age), with an average weight of 26-27 g, were included in the study and maintained in our institutional animal facility in accordance with following the animal care guidelines. The mice were randomized into five experimental groups (6 per group). Twenty-four mice were selected for the (CDX) model, and one group served as the negative control that didn’t receive any treatment. MDA-MB-231 cells (2 × 106 in 50 μL PBS & 50 μL Matrigel) were injected subcutaneously into the neck region of the mice. The mice were allowed to reach a tumor volume of 150-200 mm3 before further treatment. Then, tumor-bearing mice were randomized into the following groups: untreated xenografts (positive controls) and three treatment groups, DOX, 5-FU, and a combination of DOX and 5-FU. The mice in the DOX group were administered 1mg/kg of DOX once weekly , and the mice in the 5-FU group received 50 mg/kg of 5-FU daily for five consecutive days both treatments administered via an intraperitoneal (i.p.) route. The mice in the combination therapy group received DOX & 5-FU as separate injections, and the treatment duration was two weeks. An overview of the experiment flow. The body weight of the mice was measured at the start of the study and twice weekly from the beginning of the treatments. Tumor growth and progression were monitored by palpation and measurement of tumor size periodically using a digital vernier caliper. The tumor volume in cm3 was determined using the formula (volume = π/6 × length × width2). All mice were anesthetized and euthanized via cardiac puncture at the end of the treatment period. Tumors were excised and weighed, and their sizes were measured. Also, blood serum was collected from each mouse. Both tumor and serum samples were stored at -80°C for further analysis. |
| Sample Type: | Blood (plasma) |
