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MB Sample ID: SA323523
| Local Sample ID: | R_C24h_EA_22_1_2 |
| Subject ID: | SU003095 |
| Subject Type: | Cultured cells |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Genotype Strain: | MDA-MB-231 cells |
| Gender: | Not applicable |
| Cell Biosource Or Supplier: | ATCC (Manassas, VA, USA); ATCC HTB-26 |
| Cell Strain Details: | Epithelial breast cancer cells; absence of estrogen and progesterone receptors, HER2 overexpression |
| Cell Passage Number: | Inferior to 10 |
| Cell Counts: | 5 M |
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Collection:
| Collection ID: | CO003088 |
| Collection Summary: | MDA-MB-231 parental and MDA-MB-231/R cDDP-resistant cell lines were seeded at a density of 3 × 10^4 cells/cm2 onto 13.55 cm diameter Petri dishes, cultured in a humidified atmosphere of 5% CO2 at 37 ◦C and allowed to adhere for 24 h (t = 0 h). After this, the experiment was initiated by adding stock solutions of each drug to achieve the corresponding half of maximal inhibitory effect IC50 values: 1.0 µM cDDP and 7.9 μM Pd2Spm. Phosphate-buffer saline was used in control groups. Then, cells were incubated and collected at t = 24 h and t = 48 h, with basis on the population (25.5 ± 0.9 h and 30.6 ± 1.1 h for MDA-MB-231 and MDA-MB-231/R cells, respectively). At each time-point, cells were harvested using a 0.25% (v/v) trypsin-EDTA solution, washed twice with PBS and centrifuged (300 g, 5 min, 20 ◦C). The cell pellet was directly stored at − 80 ◦C until analysis. Three independent experiments with triplicates were performed for each cell type and time-point. |
| Sample Type: | Epithelial cells |
| Collection Method: | Trypsinization for cells harvesting followed by pellet collection and storage at − 80 ◦C (until extract prepatation) |
| Collection Frequency: | One collection per sample replicate |
| Collection Duration: | Between 2 and 5 minutes |
| Storage Conditions: | -80℃ |
