Return to study ST003064 main page
MB Sample ID: SA331979
| Local Sample ID: | R_cont_3 |
| Subject ID: | SU003179 |
| Subject Type: | Plant |
| Subject Species: | Amaranthus caudatus L. |
| Taxonomy ID: | 3567 |
| Gender: | Not applicable |
Select appropriate tab below to view additional metadata details:
Collection:
| Collection ID: | CO003172 |
| Collection Summary: | Amaranthus caudatus L., variety Karwa dauta plants were used in the study. After two weeks culturing in the hydroponic system (nutrient solution (in mmol/L) was as follows: Ca(NO3)2·4H2O - 3.81; KNO3 - 6.44; MgSO4·7H2O - 0.81; KH2PO4 - 1.83; NH4NO3 - 0.87; Fe-EDTA - 0.09; H3BO3 - 0.047; MnSO4·5H2O - 0.007; ZnSO4·7H2O - 0.0007; CuSO4·5H2O - 0.0008; (NH4)2MoO4 - 0.0005), the vessels with six-week-old plants (experimental group) were subjected to Zn2+ stress for one week which was accomplished by supplementation of 300 µmol/L ZnSO4·7H2O in the nutrient solution. Control plants remained untreated. Roots, young and mature leaves of seven-week-old Zn-treated and control plants were collected separately. Approximately 10 and 20 mg of ground dry leaf and root material, respectively, were extracted with 1 mL methanol. After vortexing (3000 g, 30 s) and centrifugation (12000 g, 4 °C, 10 min) of the suspensions, the resulted supernatants were collected. The plant material residues were additionally supplemented with 0.1 mL of deionized water. After a following vortex and centrifugation cycle, the obtained supernatants were combined with the first portions. The total extract volume was 1090 μL. Aliquots (30 μL) of the resulted aq. methanolic extracts were freeze-dried under reduced pressure with Labconco CentriVap centrifugal concentrator. The residues were sequentially derivatized with methoxyamine hydrochloride in pyridine, and N-methyl-N-(trimethylsilyl) trifluoroacetamide (MSTFA) according to the established procedure (Leonova et al., 2020, http://dx.doi.org/10.3390/ijms21020567) |
| Sample Type: | Plant leaves and roots |
