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MB Sample ID: SA332095
| Local Sample ID: | C5 |
| Subject ID: | SU003183 |
| Subject Type: | Cultured cells |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Genotype Strain: | AGS and AZ-521 |
| Species Group: | AGS and AZ-521 |
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Collection:
| Collection ID: | CO003176 |
| Collection Summary: | AGS cells or AZ-521 cells were cultured in Roswell Park Memorial Institute (RPMI) 1640 medium containing 10% fetal bovine serum, or minimal essential medium (MEM) containing 10% fetal bovine serum and 5% nonessential amino acids, respectively. AGS cells were seeded at 2x104 cells/well into 96-well plates and incubated overnight. Cultured cells were then incubated with 20 ug/mL purified VacA [activated with by addition of HCl to a pH of 3] in medium supplemented with 5 mM NH4Cl. Following intoxication, the media was removed, and cells were washed with PBS. Cells were detached by incubation with trypsin for 5 minutes and collected via centrifugation at 4°C at 1,000 rpm for 4 minutes. Trypsin was removed, cells were once again washed with PBS, and the cells were then flash-frozen in liquid nitrogen and stored at -70°C. |
| Collection Protocol Filename: | Cell_Culture_Methods.pdf |
| Sample Type: | Cultured cells |
| Storage Conditions: | -80℃ |
