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MB Sample ID: SA343036
Local Sample ID: | D26 19BBz_3 |
Subject ID: | SU003286 |
Subject Type: | Cultured cells |
Subject Species: | Homo sapiens |
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Collection:
Collection ID: | CO003279 |
Collection Summary: | Human CD8 cells were isolated with positive selection as described. The CD8 cells were activated with anti-CD3/anti-CD28 beads (Dynabeads™ Human T-Expander CD3/CD28, Gibco), as previously described107. After 48h activation, the CD8 cells were spun (1000xg for 2h at 32C) with lentiviral supernatant containing CD19-BBz/shMCJ-1 CAR, CD19-BBz/shMCJ-2 CAR or CD19-BBz/shMCJ-2 CAR construct-packing virus with rhIL-2 (40 IU/ml) and protamine sulfate. After transduction, the anti-CD3/anti-CD28 beads were removed and CD8 cells were expanded with rhIL-2 (100 IU/ml) for the specified number of days. |
Sample Type: | T-cells |