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MB Sample ID: SA343069
| Local Sample ID: | 1 |
| Subject ID: | SU003288 |
| Subject Type: | Cultured cells |
| Subject Species: | Mus musculus |
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Collection:
| Collection ID: | CO003281 |
| Collection Summary: | The E2a cells (106) were cultured in CMM for no more than 2 passages before intravenous tail vail injection to B6 mice 3 days before CAR-T cell injection. The E2a-bearing mice were irradiated with 500cGy sublethal dosage the day before CAR-T cell injection. The murine CD8 CAR-T cells were generated and expanded with rhIL-2 (60 IU/ml) as described above. The expanded CD8 CAR-T cells were washed with PBS, and 106 CAR+ cells were injected into the E2a-bearing mice via intravenous tail vail injection. The mice were followed for survival with the humane end point of development of hind limb paralysis, or weight loss of more than 20% of the original weight, or signs of major discomfort as determined by the institutional veterinarian. |
| Sample Type: | T-cells |
