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MB Sample ID: SA343139
Local Sample ID: | 20230216-M-GL-LJ-POS7-1 |
Subject ID: | SU003291 |
Subject Type: | Plant |
Subject Species: | Chili Pepper (Capsicum Chinense) |
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Collection:
Collection ID: | CO003284 |
Collection Summary: | Metabolic profiling data from chili peppers (Capsicum chinese L.) was collected. Pepper seedlings were grown in a greenhouse of Peking University Institute of Advanced Agricultural Sciences with a controlled environment of 25°C temperature, a light-dark cycle of 16 hours light and 8 hours dark, and 70% relative humidity. The fruits of peppers were sampled at seven distinct time points, starting from the day of flowering, i.e. 0 day post-anthesis (DPA) during which flowers were collected. Following this, fruits were harvested on days 7, 16, 30, 50, 55, and 60 DPA. The samples were ground and freeze-dried in liquid nitrogen, followed by extraction using 1.0 mL of 70% aqueous methanol for every 50 mg of the sample, and an ultrasonic process was employed for 30 minutes. The preparation of standards was conducted as follows: a mixed standard in the range of 20-50μg/mL was prepared using methanol of MS grade. For the amino acid standard solution, a 1mg/mL stock solution was prepared in water and subsequently diluted with 50% methanol to achieve a concentration of 50μg/mL. Three biological replicates were used metabolome analysis. |
Sample Type: | Chili Pepper (Capsicum Chinense) |