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MB Sample ID: SA353618
| Local Sample ID: | DUKE-17597 |
| Subject ID: | SU003373 |
| Subject Type: | Mammal |
| Subject Species: | Bos taurus |
| Taxonomy ID: | 9913 |
| Age Or Age Range: | 18-27 months |
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Collection:
| Collection ID: | CO003366 |
| Collection Summary: | All cattle were processed in USDA-inspected slaughter facilities and the researchers worked with the producers to collect meat samples (Pectoralis profundus) from 18 individual animals (n=18) per group for both the grass-fed and grain-fed group. Upon arrival at the lab, all meat samples were stored in a -40°C freezer and processed for analysis within 3 weeks of arrival. The meat samples were ground individually in a commercial meat grinder, and patties (112g) were cooked in a commercial oven (175 °C) until the internal temperature of the patties registered at 71 °C as determined by a meat thermometer. Thereafter ~2 grams from the center of each patty were obtained (n = 18 pasture-finished beef; n = 18 for grain-finished beef), immediately frozen in liquid nitrogen, and stored at -80 degrees °C until further analysis. |
| Sample Type: | Muscle |
| Collection Method: | Shipment on dry ice |
| Collection Location: | Columbus MT and Aberdeen, SD |
| Collection Frequency: | Once |
| Storage Conditions: | -80℃ |
| Collection Vials: | Vacuum-sealed bags |
| Storage Vials: | Cryovials |
