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MB Sample ID: SA353636
Local Sample ID: | DUKE-17598 |
Subject ID: | SU003373 |
Subject Type: | Mammal |
Subject Species: | Bos taurus |
Taxonomy ID: | 9913 |
Age Or Age Range: | 18-27 months |
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Collection:
Collection ID: | CO003366 |
Collection Summary: | All cattle were processed in USDA-inspected slaughter facilities and the researchers worked with the producers to collect meat samples (Pectoralis profundus) from 18 individual animals (n=18) per group for both the grass-fed and grain-fed group. Upon arrival at the lab, all meat samples were stored in a -40°C freezer and processed for analysis within 3 weeks of arrival. The meat samples were ground individually in a commercial meat grinder, and patties (112g) were cooked in a commercial oven (175 °C) until the internal temperature of the patties registered at 71 °C as determined by a meat thermometer. Thereafter ~2 grams from the center of each patty were obtained (n = 18 pasture-finished beef; n = 18 for grain-finished beef), immediately frozen in liquid nitrogen, and stored at -80 degrees °C until further analysis. |
Sample Type: | Muscle |
Collection Method: | Shipment on dry ice |
Collection Location: | Columbus MT and Aberdeen, SD |
Collection Frequency: | Once |
Storage Conditions: | -80℃ |
Collection Vials: | Vacuum-sealed bags |
Storage Vials: | Cryovials |