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MB Sample ID: SA354283
| Local Sample ID: | WT1 |
| Subject ID: | SU003385 |
| Subject Type: | Cultured cells |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
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Collection:
| Collection ID: | CO003378 |
| Collection Summary: | Mouse CD8+ T cells were isolated from the spleen and lymph nodes of ME2 wild-type and ME2 knockout mice (knockdown of ME2 in T cells). The mice were euthanized and soaked in 75% alcohol for 3 minutes. Separate mouse spleens using a dissector and grind them into single-cell suspensions using a grinder. Single-cell suspension was used for CD8+ T cell isolation using the Mouse Lymphocyte Isolation Kit (Biolegend, 480044, China). T cells were cultured in RMPI-1640 medium supplemented with 10% FBS, 10μg/ml β-mercaptoethanol, and 1% penicillin and streptomycin. For metabolite analysis, CD8+ T cells were stimulated with 4 μg/ml anti-CD3 and 1μg/ml anti-CD28 antibodies for 24 h. |
| Sample Type: | T-cells |
| Storage Conditions: | On ice |
