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MB Sample ID: SA355272
Local Sample ID: | pyr_2_4hr |
Subject ID: | SU003402 |
Subject Type: | Cultured cells |
Subject Species: | Bos taurus |
Taxonomy ID: | 9913 |
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Collection:
Collection ID: | CO003395 |
Collection Summary: | Cell culture media was removed and the cells were rinsed 3x with PBS using an 8-channel pipette. Lysing solution (100µL of 80/20 ACN/H2O)) was added and the cells were put over ice. The wells were flush-mixed 10 times, sonicated for 5 minutes, then centrifuged at 3,400 rpm for 10 minutes at 10ºC. The supernatant was then transferred to a 96-well plate containing 200µg of pre-aliquoted tag in each well. |
Sample Type: | Endothelial cells |