Summary of Study ST000165
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000143. The data can be accessed directly via it's Project DOI: 10.21228/M8GP4N This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
Download additional data: the amino acids were measured under negative ion chemical ionisation conditions using isobutane as reactant gas
| Study ID | ST000165 |
| Study Title | Sparing of muscle mass and function by passive loading in an experimental intensive care unit model |
| Study Type | time course + intervention |
| Study Summary | A unique experimental rat ICU model has been used allowing long-term (weeks) time-resolved analyses of the effects of standardized unilateral passive mechanical loading on skeletal muscle size and function and underlying mechanisms. Results show that passive mechanical loading alleviated the muscle wasting and the loss of force-generation associated with the ICU intervention, resulting in a doubling of the functional capacity of the loaded versus the unloaded muscles after a 2-week ICU intervention. |
| Institute | Uppsala University |
| Department | Department of Neuroscience |
| Last Name | Larsson |
| First Name | Lars |
| Lars.larsson@neuro.uu.se | |
| Submit Date | 2015-05-14 |
| Num Groups | 2 |
| Total Subjects | 13 |
| Raw Data Available | No |
| Analysis Type Detail | IR-MS |
| Release Date | 2015-05-10 |
| Release Version | 1 |
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Collection:
| Collection ID: | CO000170 |
| Collection Summary: | The tibialis anterior (TA), extensor digitorum longus (EDL), plantaris, gastrocnemius and soleus muscles were dissected from the loaded left leg and the unloaded right leg immediately after death. One half of the soleus and EDL muscles together with TA and gastrocnemius were quickly frozen in liquid propane cooled by liquid nitrogen, and stored at ?160°C for further analyses. In the other halves of the soleus and EDL muscles, bundles of approximately 50 fibres were dissected from the muscles in relaxing solution at 4°C and tied to glass capillaries, stretched to about 110% of their resting slack length. The bundles were chemically skinned for 24 h in relaxing solution containing 50% (v/v) glycerol for 24 h at 4°C and were subsequently stored at ?20°C (Larsson & Moss, 1993). All the bundles were cryo-protected within 1 week after skinning by transferring the bundles every 30 min to relax solution containing increasing concentrations of sucrose, i.e. 0, 0.5, 1.0, 1.5 and 2.0 m, and subsequently frozen in liquid propane chilled with liquid nitrogen (Frontera & Larsson, 1997). The frozen bundles were stored at ?160°C pending use. One day before the experiments, a bundle was transferred to a 2.0 m sucrose solution for 30 min, subsequently incubated in solutions of decreasing sucrose concentration (1.50.5 m) and finally kept in a skinning solution at ?20°C. |
| Sample Type: | Muscle |
