Summary of Study ST000543
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000397. The data can be accessed directly via it's Project DOI: 10.21228/M8R013 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
Study ID | ST000543 |
Study Title | High Resolution orbittrap Mass Spectrometer to measure low abundance isotope enrichment in individual muscle proteins |
Study Type | isotope encrichment and comparison of mass spectrometer platforms |
Study Summary | Stable isotope-labeled amino acids have long been used to measure the fractional synthesis rate of proteins, although the mass spectrometry platforms used for such analyses have changed throughout the years. More recently, tandem mass spectrometers such as triple quadrupoles have been accepted as the standard platform for enrichment measurement due to their sensitivity and the enhanced specificity offered by multiple reaction monitoring (MRM) experiments. The limit in the utility of such platforms for enrichment analysis occurs when measuring very low levels of enrichment from small amounts of sample, particularly proteins isolated from two-dimensional gel electrophoresis (2D-GE), where interference from contaminant ions impact the sensitivity of the measurement. We therefore applied a high resolution orbitrap mass spectrometer to the analysis of [ring-13C6]-phenylalanine enrichment in individual muscle proteins isolated with 2D-GE. Comparison of samples analyzed on both platforms revealed that the high resolution MS has significantly improved sensitivity relative to the triple quadrupole MS at very low-level enrichments due to its ability to resolve interferences in the m/z dimension. |
Institute | Mayo Clinic |
Department | Endocrinology |
Laboratory | Mayo Clinic Metabolomics Resource Core |
Last Name | Nair |
First Name | Sreekumaran |
Address | 200 First Street SW, Rochester, MN 55905 |
Nair.K@mayo.edu | |
Phone | 507-285-2415 |
Submit Date | 2016-07-22 |
Analysis Type Detail | LC-MS |
Release Date | 2017-07-10 |
Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Collection:
Collection ID: | CO000559 |
Collection Summary: | Percutaneous needle biopsies of the vastus lateralis muscle were performed under local anesthesia at 180 min and 480 min into the infusion [3;21]. The studies were repeated 65-80 days following the first study. Explanation of study design factors: Study A is the first study period, Study B is the second study conducted after 655-80 days after the first, Time 180 is the first muscle biopsy within the given study period, Time 480 is the second muscle biopsy within the given study period. Study C is the third study conducted after the second for a few subjects. |
Sample Type: | Muscle |