Summary of Study ST001305
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000886. The data can be accessed directly via it's Project DOI: 10.21228/M85D77 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
Study ID | ST001305 |
Study Title | Integrated Metabolomics and Transcriptomics Suggest the Global Metabolic Response to 2-Aminoacrylate Stress in Salmonella enterica |
Study Summary | NMR metabolomics of bacterial extractions from WT and 2-iminobutanoate/2iminopropanoate deaminase (RidA) KO S. Enterica lines |
Institute | University of Georgia |
Department | CCRC |
Last Name | Edison |
First Name | Arthur |
Address | 315 Riverbend Road, Athens, GA 30602 |
aedison@uga.edu | |
Phone | 7065428156 |
Submit Date | 2020-01-09 |
Num Groups | 2 |
Total Subjects | 19 |
Raw Data Available | Yes |
Analysis Type Detail | NMR |
Release Date | 2020-03-03 |
Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Collection:
Collection ID: | CO001374 |
Collection Summary: | Ten biologically independent cultures each of wild-type (DM9404) and ridA mutant (DM3480) strains were grown overnight in NB medium at 37 °C and used to inoculate (1% inoculum) 250 mL minimal glucose medium in 500 mL non-baffled flasks. Flasks were randomly arranged in an Innova®44 incubator and cultures were allowed to grow 16 h shaking at 180 RPM and 37 °C. Cultures were cooled on ice 5 min and then harvested by centrifugation at 7,000 x G for 10 min at 4 °C. The supernatant was decanted, pellets were resuspended in 10 mL ddH2O and transferred to sterile 15 mL conical tubes in which they were pelleted at 7,000 x G 10 min at 4 °C. Final supernatant was decanted and pellets were frozen in liquid nitrogen and stored at -80 °C prior to cell extractions. |
Sample Type: | Bacterial cells |
Storage Conditions: | -80℃ |