Summary of Study ST001738
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001112. The data can be accessed directly via it's Project DOI: 10.21228/M8ZM49 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
Study ID | ST001738 |
Study Title | AdipoAtlas: A Reference Lipidome for Human White Adipose Tissue |
Study Summary | Obesity, characterized by expansion and metabolic dysregulation of white adipose tissue (WAT), has reached pandemic proportions and acts as a primer for a wide range of metabolic disorders. Remodelling of WAT lipidome in obesity and associated comorbidities can explain disease etiology and provide valuable diagnostic and prognostic markers. To support understanding of WAT lipidome remodelling at molecular level, we performed in-depth lipidomics profiling of human subcutaneous and visceral WAT of lean and obese individuals. Tissue-tailored preanalytical and analytical workflows allowed accurate identification and semi-absolute quantification of 1636 and 737 lipid molecular species, respectively, and summarized here in a form of human WAT reference lipidome. Deep lipidomic profiling allowed to identify main lipid (sub)classes undergoing depot/phenotype specific remodelling. Furthermore, previously unanticipated diversity of WAT ceramides was uncovered. AdipoAtlas reference lipidome will serve as a data-rich resource for development of WAT-specific high-throughput methods and as a scaffold for systems medicine data integration. |
Institute | University of Leipzig |
Department | Faculty for Chemistry and Mineralogy, Biotechnological-Biomedical Center |
Laboratory | Fedorova Lab |
Last Name | Fedorova |
First Name | Maria |
Address | Deutscher Platz 5 |
maria.fedorova@bbz.uni-leipzig.de | |
Phone | 03419731336 |
Submit Date | 2021-01-11 |
Num Groups | 4 |
Study Comments | Pools of subcutaneous and visceral white adipose tissue were generated from lean patients (BMI < 25; n=5) and obese (BMI > 40; n=81) |
Publications | https://doi.org/10.1016/j.xcrm.2021.100407 |
Raw Data Available | Yes |
Raw Data File Type(s) | mzML |
Analysis Type Detail | LC-MS |
Release Date | 2021-04-23 |
Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Collection:
Collection ID: | CO001808 |
Collection Summary: | Samples of human white adipose tissue from a total of 86 donors were kindly provided by Matthias Blüher as a part of Leipzig Obesity BioBank. Tissue collection was approved by the Ethics committee of the University of Leipzig (approval number: 159-12-21052012) and all subjects gave written informed consent before taking part in the study. Removed tissue samples were flash frozen in liquid nitrogen and stored at -80°C until further analysis. For the purpose of this study, we included adipose tissue samples from abdominal visceral (VAT) and subcutaneous (SAT) fat depots of lean (BMI < 25kg/m²; n = 5) and obese (BMI > 40kg/m²; n = 81) individuals. Representative tissue pools were generated according to depot and phenotype specificity. |
Sample Type: | Adipose tissue |
Collection Location: | Leipzig, Germany |
Storage Conditions: | -80℃ |
Storage Vials: | Plastic tubes |
Additives: | none |