Summary of Study ST001779
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001132. The data can be accessed directly via it's Project DOI: 10.21228/M8CQ5F This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
Study ID | ST001779 |
Study Title | Untargeted Metabolomics analysis of A549 treated with 0.5 mM extracellular ATP and 10 ng/ml TGF-beta |
Study Type | Untargeted metabolomics analysis in lung cancer cells |
Study Summary | Control, 0.5 mM extracellular ATP and 10 ng/ml TGF-beta were used to treated 5 million A549 lung cancer cells in vitro for 2, 6 and 12 hours. The untargeted metabolomics analysis was performed on the cell lysates. The main objective of the study was to determine changes in metabolite abundances in lung cancer after treatment with extracellular ATP and TGF-beta (a known EMT inducer). |
Institute | Ohio University |
Department | Biological Sciences |
Laboratory | Dr. Xiaozhuo Chen, Edison biotechnology Institute |
Last Name | Shriwas |
First Name | Pratik |
Address | Room 425, Parks Hall, College of Pharmacy, Ohio State University, Columbus Ohio. 43210 |
ps774614@ohio.edu | |
Phone | 7406033801 |
Submit Date | 2021-04-04 |
Num Groups | 7 |
Raw Data Available | Yes |
Raw Data File Type(s) | mzXML |
Analysis Type Detail | LC-MS |
Release Date | 2021-05-21 |
Release Version | 1 |
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Collection:
Collection ID: | CO001849 |
Collection Summary: | A549 lung cancer cells were treated with control, ATP, TGF-beta and then collected according to sample preparation protocol. |
Collection Protocol Filename: | Collection protocol |
Sample Type: | Lung |
Collection Method: | Ice cold methanol (80%) using cell scrappers |
Collection Location: | 150mm dish |
Collection Frequency: | Once |
Collection Duration: | 1-2 sec |
Volumeoramount Collected: | 1 ml |
Storage Conditions: | -80℃ |
Collection Vials: | Polypropylene 1.5 ml tubes |
Storage Vials: | Polypropylene 1.5 ml |
Collection Tube Temp: | 4C |
Tissue Cell Quantity Taken: | 5 million |