Summary of Study ST002071
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001313. The data can be accessed directly via it's Project DOI: 10.21228/M80H5Z This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
Study ID | ST002071 |
Study Title | Metabolic profiling of mouse CD27+ and CD27- gammadelta T cells |
Study Summary | Cultured CD27+ and CD27- gammadelta T cells were labeled with 13C-glucose for 24 h. Cells were harvested for LC-MS analysis |
Institute | University of Louisville |
Last Name | Zhang |
First Name | Xiang |
Address | Shumaker Research Bld 349, Louisville, KY 40292 |
xiang.zhang@louisville.edu | |
Phone | 502-852-8878 |
Submit Date | 2022-01-07 |
Raw Data Available | Yes |
Raw Data File Type(s) | raw(Thermo) |
Analysis Type Detail | LC-MS |
Release Date | 2022-02-14 |
Release Version | 1 |
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Collection:
Collection ID: | CO002146 |
Collection Summary: | polarized CD27+ and CD27- γδ T cells were cultured in glucose-free DMEM supplemented with 10 mM [U-13C]-glucose and 10% dialyzed FBS for 24 h. The cells were rinsed in cold PBS and quenched using a mixture containing 2 mL acetonitrile and 1.5 mL H2O. After adding 1 mL chloroform, the sample was homogenized and centrifuged at 3,000 rpm, 4 oC for 20 min. The top layer was transferred to a new tube and lyophilized. The dried sample was then dissolved in 100 µL 20% acetonitrile and vigorously vortex-mixed for 3 min. After centrifugation at 14,000 rpm, 4oC for 20 min, the supernatant was collected for two-dimensional liquid chromatography-mass spectrometry (2DLC-MS) analysis. |
Sample Type: | T-cells |